Bacterial protein degradation by different rumen protozoal groups1

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Bacterial protein degradation by different rumen protozoal groups1

© 2012 American Society of Animal Science. All rights reserved. J. Anim. Sci. 2012.90:4495–4504 doi:10.2527/jas2012-5118

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Bacterial protein degradation by different rumen protozoal groups.

Bacterial predation by protozoa has the most deleterious effect on the efficiency of N use within the rumen, but differences in activity among protozoal groups are not completely understood. Two in vitro experiments were conducted to identify the protozoal groups more closely related with rumen N metabolism. Rumen protozoa were harvested from cattle and 7 protozoal fractions were generated imme...

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Relation of rumen ATP concentration to bacterial and protozoal numbers.

Cultures of Streptococcus bovis and mixed populations of rumen bacteria were used to investigate the concentration of ATP and rumen bacterial numbers at various stages of growth. ATP, extracted with Tris buffer, was analyzed using the firefly luciferin-luciferase bioluminescent reaction. ATP concentrations of S. bovis and mixed cultures of rumen bacteria significantly correlated with live cell ...

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Rumen Bacterial and Protozoal Responses to Insecticide Substrates.

Insecticides containing organophosphate, chlorinated hydrocarbon, and carbamate were tested with bovine ruminal ingesta fractions. Rumen bacteria exposed to insecticide levels of 0 to 500 ppm in rumen fluid for 4 hr were inoculated into rumen fluid-starch feed extract medium. No apparent significant bacterial count inhibitions were noted. Also, when insecticides were used as carbon sources at c...

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Ruminal Protein Degradation and Estimation of Rumen Microbial Protein Production

Animal agricultural production systems are major sources of nonpoint pollution affecting quality of water sources. Nitrogen has been identified as the foremost source of nonpoint water pollution  and the potential negative impacts of N have become an area of public concern. protein degradation from feed ingredients is an important factorinfluencing AA supply to the duodenum. Ruminal proteolysis...

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ژورنال

عنوان ژورنال: Journal of Animal Science

سال: 2012

ISSN: 0021-8812,1525-3163

DOI: 10.2527/jas.2012-5118